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As the mobile phase is always less polar than the stationary phase in normal phase tlc, polar compounds will tend to have a lesser affinity for the mobile phase than nonpolar compounds (based on the like dissolves like principle). Examples of polar solvent systems include ethyl acetate, methanol, and dichloromethane. Polar substances will interact strongly with these water molecules and move up the paper more slowly
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This means that, even if the solvent is polar, a nonpolar molecule will travel further up the tlc plate than a polar molecule due to its attraction to the polar adsorbent. They keep most nonpolar compounds closer to the starting line Polar compounds will be adsorbed more strongly onto the tlc plate and will not move as far, creating a separation.
Depending on the relative polarity of the sample and the eluent, the sample either adsorbs to the solid phase or is eluted by the mobile phase (eluent)
If the eluent is very polar relative to your compound, it will dissolve your sample and the sample will move with the mobile phase. Your compounds will travel different distances up the plate depending on the solvent you choose The very polar silica gel tries to hold the spot in its original place and the solvent tries to move the spot along with it as it travels up the plate Larger molecules take longer to move up the chromatography paper or tlc plate, whereas smaller molecules are more mobile
Likewise, the polarity of the molecules can affect how far. In thin layer chromatography (tlc), nonpolar compounds travel further because they have less affinity for the polar stationary phase and more affinity for the nonpolar mobile phase. Polar solvents prompt polar components to move further up the plate
